Which of these enzymes are not needed for DNA replication?
Ligase
Restriction endonucleases
DNA Polymerase
Why can both strands of DNA not be replicated continuously?
DNA nucleotides can only be added continuously in a 5’ to 3’ direction
The DNA polymerase can only act on one strand at a time
There is not enough space for both strands to be copied
In DNA replication, the fragments of DNA which have been replicated discontinuously are joined together using what?
PCR is a technique used for what?
The amplification of DNA in vitro
The amplification of DNA in vivo
Sequencing the nucleotide bases of DNA
What are primers?
Single stranded random sequences of nucleotides
Single stranded sequences of nucleotides which are complementary to a target sequence
Double stranded sequences of nucleotides which are complementary to a target sequence